Mating-type genes in the *Citrus*-associated *Phyllosticta* species are highly variable in their sequence content, but the genomic locations and organization of the mating-type loci are conserved. ncestral state reconstruction suggests that homothallism is the ancestral thallism state in *Phyllosticta*, with a shift to heterothallism in *Phyllosticta* species that are pathogenic to *Citrus*.
We analyzed a library of mutants obtained through *Agrobacterium tumefaciens* transformation system, looking for alterations in growth and reproductive structure formation. Two mutant strains were found to have lost the ability to form pycnidia. The predicted genes containing the T-DNA insertions were identified as Spindle Poison Sensitivity Scp3, Ion Transport protein, and Cullin Binding proteins. We suggest these genes as new targets for fungicide development and CBS disease control, by iRNA.
Desenvolvimento de novas estratégias para o controle de P. citricarpa por meio de abordagens funcionais
Análises comparativas nas espécies de Phyllosticta associadas a Citros, focando na evolução de mecanismos associados à patogenicidade em P. citricarpa